diff --git a/biology/pyfasta/Makefile b/biology/pyfasta/Makefile
index e2547c131b45..51ce599a94d8 100644
--- a/biology/pyfasta/Makefile
+++ b/biology/pyfasta/Makefile
@@ -6,7 +6,7 @@
 #
 
 PORTNAME=	pyfasta
-PORTVERSION=	0.4.0
+PORTVERSION=	0.4.1
 CATEGORIES=	biology python
 MASTER_SITES=	CHEESESHOP
 
diff --git a/biology/pyfasta/distinfo b/biology/pyfasta/distinfo
index 2692ff7a9a6d..1fe603a3b0b2 100644
--- a/biology/pyfasta/distinfo
+++ b/biology/pyfasta/distinfo
@@ -1,3 +1,2 @@
-MD5 (pyfasta-0.4.0.tar.gz) = c978ef60bf8eaeaf6c550f38c475dcb6
-SHA256 (pyfasta-0.4.0.tar.gz) = cbc356aaae4d17028d4ddba61a9af19fe89f853ec81ee82e4ad39b008e6ba33d
-SIZE (pyfasta-0.4.0.tar.gz) = 14558
+SHA256 (pyfasta-0.4.1.tar.gz) = 1f68935c8143d7a387ea04eb8461524436e4e4c940bf894d081d2e19c9bebfe4
+SIZE (pyfasta-0.4.1.tar.gz) = 14760