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Optimise combinations of primers and minimise the formation of dimers in multiplexed PCR. Primer Pooler can: * Check through each proposed pool for combinations that are likely to form dimers * Automatically move prospective amplicons between proposed pools to reduce dimer formation * Automatically search the genome sequence to find which amplicons overlap, and place their corresponding primers in separate pools * Optionally keep pool sizes within a specified range * Handle thousands of primers without being slow (useful for high-throughput sequencing applications) * Do all of the above with degenerate primers too. WWW: http://ssb22.user.srcf.net/pooler/ PR: ports/251065 Submitted by: Silas S. Brown <silas-freebsd@flatline.org.uk>
23 lines
679 B
Plaintext
23 lines
679 B
Plaintext
Optimise combinations of primers and minimise the formation of dimers in
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multiplexed PCR.
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Primer Pooler can:
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* Check through each proposed pool for combinations that are likely to form
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dimers
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* Automatically move prospective amplicons between proposed pools to reduce
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dimer formation
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* Automatically search the genome sequence to find which amplicons overlap, and
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place their corresponding primers in separate pools
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* Optionally keep pool sizes within a specified range
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* Handle thousands of primers without being slow (useful for high-throughput
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sequencing applications)
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* Do all of the above with degenerate primers too.
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WWW: http://ssb22.user.srcf.net/pooler/
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