diff --git a/nixos/tests/kubo/kubo-fuse.nix b/nixos/tests/kubo/kubo-fuse.nix
index adc1ab1b9e41..a1afd4e1eb0b 100644
--- a/nixos/tests/kubo/kubo-fuse.nix
+++ b/nixos/tests/kubo/kubo-fuse.nix
@@ -43,11 +43,39 @@
         machine.succeed(f"diff /tmp/test.txt /ipfs/{ipfs_hash}")
 
 
-    with subtest("Unmounting of /ipns and /ipfs"):
+    with subtest("/mfs/ FUSE mountpoint"):
+        with subtest("Write the test file in three different ways"):
+            machine.succeed("cp /tmp/test.txt /mfs/test-1.txt")
+            machine.succeed("su alice -c 'ipfs files write --create /test-2.txt < /tmp/test.txt'")
+            machine.succeed(f"ipfs files cp /ipfs/{ipfs_hash} /test-3.txt")
+
+        with subtest("Show the files (for debugging)"):
+            # Different hashes for the different ways of adding the file to the MFS probably come from different linking structures of the merkle tree. Copying the file to /mfs with `cp` is even non-deterministic.
+            machine.succeed("ipfs files ls --long >&2")
+            machine.succeed("ls -l /mfs >&2")
+
+        with subtest("Check that everyone has permission to read the file (because of Mounts.FuseAllowOther)"):
+            machine.succeed("su alice -c 'cat /mfs/test-1.txt' | grep fnord3")
+            machine.succeed("su bob -c 'cat /mfs/test-1.txt' | grep fnord3")
+
+        with subtest("Check the file contents"):
+            machine.succeed("diff /tmp/test.txt /mfs/test-1.txt")
+            machine.succeed("diff /tmp/test.txt /mfs/test-2.txt")
+            machine.succeed("diff /tmp/test.txt /mfs/test-3.txt")
+
+        with subtest("Check the CID extended attribute"):
+            output = machine.succeed(
+                "getfattr --only-values --name=ipfs_cid /mfs/test-3.txt"
+            ).strip()
+            assert ipfs_hash == output, f"Expected {ipfs_hash} but got {output}"
+
+
+    with subtest("Unmounting of /ipns, /ipfs and /mfs"):
         # Force Kubo to crash and wait for it to restart
         machine.systemctl("kill --signal=SIGKILL ipfs.service")
         machine.wait_for_unit("ipfs.service", timeout = 30)
 
         machine.succeed(f"diff /tmp/test.txt /ipfs/{ipfs_hash}")
+        machine.succeed("diff /tmp/test.txt /mfs/test-3.txt")
   '';
 }