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Optimise combinations of primers and minimise the formation of dimers in multiplexed PCR. Primer Pooler can: * Check through each proposed pool for combinations that are likely to form dimers * Automatically move prospective amplicons between proposed pools to reduce dimer formation * Automatically search the genome sequence to find which amplicons overlap, and place their corresponding primers in separate pools * Optionally keep pool sizes within a specified range * Handle thousands of primers without being slow (useful for high-throughput sequencing applications) * Do all of the above with degenerate primers too. WWW: http://ssb22.user.srcf.net/pooler/ PR: ports/251065 Submitted by: Silas S. Brown <silas-freebsd@flatline.org.uk>
4 lines
187 B
Plaintext
4 lines
187 B
Plaintext
TIMESTAMP = 1605447867
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SHA256 (ssb22-PrimerPooler-v1.75_GH0.tar.gz) = 7d0c0c6b58724a42100b1019995385568747f3b5620b09b297d3e77758c35818
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SIZE (ssb22-PrimerPooler-v1.75_GH0.tar.gz) = 125999
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