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Primer3 helps to choose primers for PCR reactions PR: 35298 Submitted by: Tony Maher <tonym@biolateral.com.au>
24 lines
826 B
Plaintext
24 lines
826 B
Plaintext
"Primer3 is a complete rewrite of the original PRIMER program
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(Primer 0.5), written by Steve Lincoln, Mark Daly, and Eric
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Lander. See DIFFERENCES FROM EARLIER VERSIONS for a discussion
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of how Primer3 differs from its predecessors, Primer 0.5 and
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Primer v2.
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Primer3 picks primers for PCR reactions, considering as criteria:
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o oligonucleotide melting temperature, size, GC content,
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and primer-dimer possibilities,
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o PCR product size,
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o positional constraints within the source sequence, and
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o miscellaneous other constraints.
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All of these criteria are user-specifiable as constraints, and
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some are specifiable as terms in an objective function that
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characterizes an optimal primer pair."
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- from the README file
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WWW: http://www-genome.wi.mit.edu/genome_software/other/primer3.html
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--
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Tony Maher <tonym@biolateral.com.au>
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