mirror of
https://git.FreeBSD.org/ports.git
synced 2024-11-24 00:45:52 +00:00
47be6d4a10
Primer3 helps to choose primers for PCR reactions PR: 35298 Submitted by: Tony Maher <tonym@biolateral.com.au>
24 lines
826 B
Plaintext
24 lines
826 B
Plaintext
"Primer3 is a complete rewrite of the original PRIMER program
|
|
(Primer 0.5), written by Steve Lincoln, Mark Daly, and Eric
|
|
Lander. See DIFFERENCES FROM EARLIER VERSIONS for a discussion
|
|
of how Primer3 differs from its predecessors, Primer 0.5 and
|
|
Primer v2.
|
|
|
|
Primer3 picks primers for PCR reactions, considering as criteria:
|
|
|
|
o oligonucleotide melting temperature, size, GC content,
|
|
and primer-dimer possibilities,
|
|
o PCR product size,
|
|
o positional constraints within the source sequence, and
|
|
o miscellaneous other constraints.
|
|
|
|
All of these criteria are user-specifiable as constraints, and
|
|
some are specifiable as terms in an objective function that
|
|
characterizes an optimal primer pair."
|
|
- from the README file
|
|
|
|
WWW: http://www-genome.wi.mit.edu/genome_software/other/primer3.html
|
|
|
|
--
|
|
Tony Maher <tonym@biolateral.com.au>
|